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KMID : 0616120090400040309
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Korean Journal of Pharmacognosy
2009 Volume.40 No. 4 p.309 ~ p.314
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Analysis of Liquiritigenin, an Aglycone of Liquiritin in Licorice by High Performance Liquid Chromatography
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Lee Jong-Hwa
Ze Keum-Ryon
Kim Do-Hoon
Park Ju-Young
Shim Young-Hoon
Kim Jong-Hwan
Lim Sook
Shin Jin-Seon
Kim In-Seon
Kim Ji-Yeon
Sung Sang-Hyun
Jang Seung-Yeup
Kim Dong-Seup
Seong Rack-Seon
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Abstract
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Licorice(Glycyrrhizae Radix et Rhizoma) is recorded as the root of Glycyrrhiza uralensis Fischer or Glycyrrhiza
glabra Linne or Glycyrrhiza inflata Bat.(Leguminosae) in Korean Pharmacopoeia 9 thedition (KP9) and Chinese Pharmacopoeia 2005(CP2005), Glycyrrhiza uralensis Fischer or Glycyrrhiza glabra Linne in Japanese Pharmacopoeia 2005(JP2005). It is established the content standard of Glycyrrhizin 2.5 % and liquiritin 1% in KP9 and CP2005. But, according to the reports, all Licorice species were not sufficient for content standard of liquiritin 1.0% for licorice in KP9 and CP2005. It shows different content of liquiritin among G. uralensis, G. glabra and G. inflata. Also, it was reported liquiritin, liquiritin apioside are transformed into liquiritigenin by human internal flora. Therefore, we have studied for the pre-treatment condition and analytical method of liquiritigenin; It was good efficinet in 2M HCl reflux(1 hr) for hydrolysis and in methylene chloride for solvent fractionation. And 1% acetic acid in DW(A) and acetonitrile(B) with gradient condition as a mobile phase was most effective in HPLC analytical condition. According to these experimental methods, we have anlayzed content of liquiritigenin about 77 Licorice sample. In this research, it was also examined the content of liquiritin and liquiritigenin for Glycyrrhizae Radix related growing area. According to the results, we suggested the content standard of glycyrrhizin more than 2.5%, liquiritigenin more than 0.7%(after hydrolysis) of licorice.
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KEYWORD
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Licorice, Liquiritigenin, Analysis, HPLC
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